PD Dr. Christoph Westerhausen
| Telefon: | +49 821 598 - 3311 |
| E-Mail: | christoph.westerhausen@med.uni-augsburg.de |
| Raum: | 335 (R) |
| Adresse: | Universitätsstraße 1 (Physik Nord), 86159 Augsburg |
Deine Abschlussarbeit als Beitrag zu unserer Forschung?
Motivierte Studierende (Physik/Mawi/Medizin) gesucht für Abschlussarbeiten JETZT !
Du willst an unserem Projekt “Physical and functional interaction mechanisms at cell membranes and vessel walls“ mitwirken in Form einer/eines
- Bachelorarbeit
- Fachpraktikums
- Projektarbeit
- Laboraty Projects
- Masterarbeit
- Medizinischen Doktorarbeit?
Dann kontaktiere uns jetzt! => christoph.westerhausen@physik.uni-augsburg.de
Forschungsschwerpunkte
Cell manipulation, stimulation and sensing
• Acoustical stimulation of cell proliferation and migration
• Cell adhesion under flow
• Neural networks
• Acoustic Live Cell Sensing of wound healing, migration and adhesion
Thermodynamics of membranes and living systems
• Sound and shear enhanced drug uptake in cells
• Nanoparticle uptake in cells and lipid vesicles
• Correlation of membrane state and function (e.g. enzyme activity or uptake)
Cell-cell interaction
• Dynamics and stability of red blood cell aggregates (rosettes)
• Cell adhesion (endothelial cells, WBC, melanoma cells)
Technology and Biomaterials
• Wear resist and antibacterial diamond-like carbon surface modifications for implants
• Fast mixing at low Reynolds numbers for creation of therapeutic nanoparticles
• Acoustophotocatalysis - Employing SAW in heterogeneous catalysis
News
>> Outreach: On June 28th at 6 pm we present our research topics at the "Lehrstuhlabend" to all interested students with talks and lab tours, starting in the physics lecture hall. We are looking forward to meeting you!
>> Research: Congratulations Nico, Jonas and Andrej! Our paper "Shear stress induced lipid order and permeability changes of giant unilamellar vesicles" has been accepted and will appear soon in BBA General Subjects!
Key findings:
- Membrane permeability increase by transition from gel to fluid phase and vice versa
- All-or-nothing GUV permeabilization under shear flow near the phase transition
- Gradual GUV permeabilization under shear flow far off the phase transition
- Phase state dependent membrane permeability increase induced by shear forces
- Lipid membrane melting temperature shift induced by shear forces
>> Research: Congratulations Kathrin, Sebastian, Sophie and Manuel! Our paper "One-dimensional acoustic potential landscapes guide the neurite outgrowth and affect the viability of B35 neuroblastoma cells" appeared as accepted manuscript in Physical Biology
https://iopscience.iop.org/article/10.1088/1478-3975/ac70a1 !
Key findings:
- applicable SSAW power levels can be increased by employing pulsed rf signals
- B35 neurite orientation is influenced by acoustic potential landscapes
- proliferation in microchannels is decreased compared to standard culture
- with increasing acoustic force the proliferation is further decreased (≈180 nN per cell => relative proliferation rate ≈ 50%)
- mild acoustic forces up to ≈ 100 nN do not reduce or slightly improve viability, while higher forces reduce the viability (measured as relative time to apoptosis )
>> Research: Our contribution „Handy Nanoquakes“ on the facile manipulation of micrometre-sized objects and living cells appeared today in Nature Materials https://www.nature.com/articles/s41563-022-01236-y.
>> Research: Congratulations! Our paper “Directed invasion of cancer cell spheroids inside 3D collagen matrices oriented by microfluidic flow in experiment and simulation" in collaboration with the Engelke group (Graz) has been accepted and will appear soon in PLOS ONE.
>> Research: Congratulations! Lukas’ paper “Reversible single cell trapping of Paramecium caudatum to correlate swimming behavior and membrane state" in collaboration with the Schneider group (TU Dortmund) has been accepted and will appear soon in Biomicrofluidics.
>> Research: Congratulations! Lukas’ and Kathi’s paper “Acetylcholinesterase Activity Influenced by Lipid Membrane Area and Surface Acoustic Waves" appeared in the Special Issue „Lipid Bilayers on Chip, Volume II“ in Micromachines.
https://www.mdpi.com/2072-666X/13/2/287
Key points:
- the patterning of supported lipid membranes with SAW is semi-reversible with two diffusion time scales
- the modulation of the activity of Acetylcholinesterase bound to Lipid Membranes in standing SAW fields is low
- the activity of AChE is modulated by the available membrane area
>> Research: Congratulations! Andrej´ s paper “The activity of the intrinsically water-soluble enzyme ADAMTS13 correlates with the membrane state when bound to a phospholipid bilayer" is accepted and will appear in Scientific Reports soon!
Key points:
- the temperature dependence of the activity of ADAMTS13 when bound to a lipid bilayer shows a distinct maximum close to the main phase transition temperature Tm of the membrane
- changing the carbon chain length of the used lipid, and thus Tm, shifts the activity maximum to the new Tm
- comparing the activity at a constant temperature for three different membranes in the gel phase, the fluid phase and at the transition underline the role of the membrane state for the enzyme activity
- these results are perfectly in line with the concept that increased fluctuations close to a phase transition are crucial for catalysis
>> Teaching: New elective starting next summer term: „Physiology in everyday life“ - your idea/research question, your implementation, your answer!
>> Award: We got the CeNS publication award 2021 in the category „Best Junior Scientist Publication“ for our paper Vibration enhanced cell growth induced by surface acoustic waves as in vitro wound healing model, PNAS!
>> Research: Congratulations! Anna´´ s and Simon´ s paper “Dynamic Effective Elasticity of Melanoma Cells under Shear and Elongational Flow Confirms Estimation from Force Spectroscopy” is accepted and will appeared in Biophysica.
article: https://www.mdpi.com/2673-4125/1/4/32/htm.
Key points:
- The elasticity of A375 melanoma cells was determined by applying different methods: AFM + a cantilever with a pyramidal tip, AFM + tipless cantilevers and deformation analysis during passing a stenosis in a micro channe.l
- We found higher and more widely distributed Young´´ s moduli E using a cantilever with a tip compared with the other methods due to local variations of E.
- E scales with the logarithm of the loading rate.
- The effective, overall elasticity indicates two population of the studied cell line.
>> Research: Congratulations!Nico´´ s paper “Broad lipid phase transitions in mammalian cell membranes measured by Laurdan fluorescence spectroscopy” is accepted and will appear soon in BBA Biomembranes.
preprint: https://www.researchsquare.com/article/rs-515297/v1
article (proofreading pending): https://www.sciencedirect.com/science/article/abs/pii/S000527362100242X
Key points:
- Membrane embedded Laurdan displays order disorder phase transitions even in frozen water
- Cell membranes undergo order-disorder phase transitions as lipid vesicles but over a broader temperature range
- As known for lipid vesicles order-disorder phase transitions in cell membranes are sensitive to cholesterol content, anaesthetic and pH
- Order-disorder phase transitions in cell membranes are influenced by cell adaption processes, morphological changes and protein content
>> Research: Congratulations! Andrej´´`s and Felix´´ s paper “Transient permeabilization of living cells combining shear flow and acoustic trapping for facilitated uptake of molecules” is accepted and will appeared soon in the Special Issue Developing Biophysical Tools in Processes.
https://www.mdpi.com/2227-9717/9/6/913/htm
Key points:
- Molecular uptake under shear force @RT is a non-linear function of shear stress with a pronounced maximum, that could be related with a phase transition of the plasma membrane.
- Acoustic trapping under flow increases the uptake up to a factor of 18.
- This permeabilization method works for impermeable dyes, sugars and proteins.
>> Research: Congratulations! Sixuan´´`s paper “Surface acoustic wave enhanced water splitting reaction with methanol as sacrifice material” is accepted and will appeared soon in Catalysis Science and Technology of the RSC.
https://pubs.rsc.org/en/content/articlelanding/2021/cy/d0cy01788d/unauth#!divAbstract
Key points:
- SAW (Rayleigh waves) enhance water splitting using a Pt/TiO2 catalyst by more than +100%
- The SAW accompanying electric field is crucial
>> Research: Our paper “Shear-Horizontal Surface Acoustic Wave Sensor for Non-Invasive Monitoring of Dynamic Cell Spreading and Attachment in Wound Healing Assays” is accepted and will appear soon in Biosensors and Bioelectronics (Vol 173 (2021) 112807).
Download for free till Januar 8th 2021: https://authors.elsevier.com/a/1c62U3PVtpkTxg
Key points:
- Love waves allow for non-optical sensing of wound-healing-assays
- Changes in cell adhesion strength influence the effective permittivity
- Monitoring of culture conditions, like changes in osmolarity, is easily possible
- Cell detachment kinetics can be measured quantitatively without microscopy
>> Research: Congratulations to our team, especially Manu, Kathi and Sophie! Our paper “Vibration enhanced cell growth induced by surface acoustic waves as in vitro wound healing model” will appear soon appeared in PNAS.
https://www.pnas.org/content/early/2020/11/25/2005203117
Key points:
- SAW enhanced proliferation
- SAW enhanced migration up to +135%
- wave type is crucial (vertical substrate deflection)
- effect conserved for various cell lines
>> Funding: Nicolas Färber just has been awarded with a Add-on Fellowship for Interdisciplinary Life Science 2020 of the Joachim-Herz-Stiftung! We congratulate Nico and thank the funders!
>> Research: We recently got funding for our collaboration project „Physikalische und funktionelle Mechanismen der Interaktionen an Zell- und Gefäßwänden“ (Physical and functional interaction mechanisms at cell membranes and vessel walls)
Collaborators:
- Dr. Stefanie Grützner (Transfusionsmedizin und Hämostaseologie)
- Dr. Torsten Gloe (Physiologie)
- Prof. Dr. Udo Jeschke (Interdisziplinäres onkologisches Forschungslabor)
- Prof. Dr. Achim Wixforth (Experimentalphysik I)
- Prof. Dr. Julia Welzel (Klinik für Dermatologie und Allergologie)
https://www.uni-augsburg.de/de/fakultaet/med/forschung/forschungsfoerderung/gefoerderte-projekte/
> Research: Anna´’s paper “Blood group and size dependent stability of P. falciparum infected red blood cell aggregates in capillaries” appeared in Biomicrofluidics!
>> paper: download here https://aip.scitation.org/doi/10.1063/1.5125038
>> Research: The summary on our successful ITN SAWTrain can be found online now!
>> Research: Patta´´’s paper “Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection” just got accepted and will appeared soon in Scientific Reports!
>> paper: download here (open access) https://www.nature.com/articles/s41598-019-55110-0.pdf
>> Research: Sascha´´’s, Andrej´’s and Sven´’s paper “Smart antimicrobial efficacy employing pH-sensitive ZnO-doped DLC coatings” just got accepted and will appeared soon in Scientific Reports!
>> press release: read more https://www.uni-augsburg.de/de/campusleben/neuigkeiten/2019/11/21/1048/
>> paper: download here (open access) https://www.nature.com/articles/s41598-019-53521-7.pdf
Publikationen
Accepted and recently published
N. Färber, J. Reitler, A. Kamenac, and C. Westerhausen,
"Shear stress induced lipid order and permeability changes of giant unilamellar vesicles"
BBA General Subjects, accepted 2022
K. Baumgartner, S. C. F. Mauritz, S. Angermann, M. S. Brugger*, and C. Westerhausen*, "One-dimensional acoustic potential landscapes guide the neurite outgrowth and affect the viability of B35 neuroblastoma cells",
Physical Biology, Accepted Manuscript online 17 May 2022, 2022, *corresponding authors
H. J. Krenner, and C. Westerhausen, „Handy Nanoquakes“,
Nature Materials, 21, 499–501, 2022.
F. Geiger, L. G. Schnitzler, M. S. Brugger, C. Westerhausen*, and H. Engelke*, “ "Directed invasion of cancer cell spheroids inside 3D collagen matrices oriented by microfluidic flow in experiment and simulation",”,
PLOS ONE, Vol. 17 (3), 2022, *corresponding authors
A. M. Jötten, S. Neidinger, J.K. Tietze, J. Welzel, and C. Westerhausen, „Dynamic effective elasticity of melanoma cells under shear and elongational flow confirms estimation from force spectroscopy”,
Biophysica, Vol. 1 (4), 445-457, 2021.
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2022 |
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Lukas G. Schnitzler, Kathrin Baumgartner, Anna Kolb, Benedikt Braun und Christoph Westerhausen
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Nicolas Färber und Christoph Westerhausen
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Florian Geiger, Lukas G. Schnitzler, Manuel S. Brugger, Christoph Westerhausen und Hanna Engelke
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Hubert J. Krenner und Christoph Westerhausen
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Kathrin Baumgartner, Sophie C. F. Mauritz, Sebastian Angermann, Manuel S. Brugger und Christoph Westerhausen
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Lukas G. Schnitzler, Anne Paeger, Manuel S. Brugger, Matthias F. Schneider und Christoph Westerhausen
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2021 |
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Manuel S. Brugger, Lukas G. Schnitzler, Timo Nieberle, Achim Wixforth und Christoph Westerhausen
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Sixuan Wang, Rudolf Herrmann, Alexander Reiner, Achim Wixforth und Christoph Westerhausen
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Andrej Kamenac, Tobias Obser, Achim Wixforth, Matthias F. Schneider und Christoph Westerhausen
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Andrej Kamenac, Felix L. Schilberth, Ernst Wagner, Achim Wixforth, Ulrich Lächelt und Christoph Westerhausen
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2020 |
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Anna Martina Jötten, Kirsten Moll, Mats Wahlgren, Achim Wixforth und Christoph Westerhausen
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Lukas Bergerhausen, Julius Grosche, Juliane Meißner, Christina Hecker, Michele F. Caliandro, Christoph Westerhausen, Andrej Kamenac, Maryam Rezaei, Matthias Mörgelin, Gereon Poschmann, Dietmar Vestweber, Eva-Maria Hanschmann und Johannes A. Eble
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U. Ratzel, O. Bayer, P. Bracht, Martin Hoffmann, Kai Jänke, Karl-Jürgen Kiesel, Clemens Mehnert, Christoph Scheck, Christoph Westerhausen, K.-G. Krapf, L. Herrmann und Janosch Blaul
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Manuel S. Brugger, Kathrin Baumgartner, Sophie C. F. Mauritz, Stefan C. Gerlach, Florian Röder, Christine Schlosser, Regina Fluhrer, Achim Wixforth und Christoph Westerhausen
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2019 |
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Anna Martina Jötten, S. Angermann, Melanie E. M. Stamp, Dominik Breyer, Florian G. Strobl, Achim Wixforth und Christoph Westerhausen
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Patrick W. Kudella, Katharina Preißinger, Matthias Morasch, Christina F. Dirscherl, Dieter Braun, Achim Wixforth und Christoph Westerhausen
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Florian G. Strobl, D. M. Czubak, Achim Wixforth und Christoph Westerhausen
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Lukas G. Schnitzler, Stefanie Junger, Dominik M. Loy, Ernst Wagner, Achim Wixforth, Andreas Hörner, Ulrich Lächelt und Christoph Westerhausen
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Sascha Buchegger, Andrej Kamenac, Sven Fuchs, Rudolf Herrmann, Pia Houdek, Christian Gorzelanny, Andreas Obermeier, Stephan Heller, Rainer Burgkart, Bernd Stritzker, Achim Wixforth und Christoph Westerhausen
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Per Delsing, Andrew N. Cleland, Martin J. A. Schuetz, Johannes Knörzer, Géza Giedke, J. Ignacio Cirac, Kartik Srinivasan, Marcelo Wu, Krishna Coimbatore Balram, Christopher Bäuerle, Tristan Meunier, Christopher J. B. Ford, Paulo V. Santos, Edgar Cerda-Méndez, Hailin Wang, Hubert J. Krenner, Emeline D. S. Nysten, Matthias Weiß, Geoff R. Nash, Laura Thevenard, Catherine Gourdon, Pauline Rovillain, Max Marangolo, Jean-Yves Duquesne, Gerhard Fischerauer, Werner Ruile, Alexander Reiner, Ben Paschke, Dmytro Denysenko, Dirk Volkmer, Achim Wixforth, Henrik Bruus, Martin Wiklund, Julien Reboud, Jonathan M. Cooper, YongQing Fu, Manuel S. Brugger, Florian Rehfeldt und Christoph Westerhausen
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2018 |
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Melanie E. M. Stamp, Christoph Westerhausen, Wei Tong, Steven Prawer, David J. Garrett und Achim Wixforth
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Christoph G. Wittmann, Andrej Kamenac, Florian G. Strobl, Dietmar Czubak, Achim Wixforth und Christoph Westerhausen
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Jochen Taiber, Sascha Buchegger, Bernd Stritzker, Achim Wixforth und Christoph Westerhausen
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Manuel S. Brugger, Sarah Grundeen, Adele Doyle, Luke Theogarajan, Achim Wixforth und Christoph Westerhausen
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2017 |
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Andrej Kamenac, Achim Wixforth, T. Obser, S. Reinhard, M. F. Schneider und Christoph Westerhausen
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Mario Djukelic, Achim Wixforth und Christoph Westerhausen
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Sascha Buchegger, Natascha Schuster, Bernd Stritzker, Achim Wixforth und Christoph Westerhausen
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Manuel S. Brugger, S. Grundeen, L. Theogarajan, Achim Wixforth und Christoph Westerhausen
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Anna Martina Jötten, T. Geislinger, K. Moll, M. Wahlgren, Achim Wixforth und Christoph Westerhausen
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2016 |
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Patrick W. Kudella, Kirsten Moll, Mats Wahlgren, Achim Wixforth und Christoph Westerhausen
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Melanie E. M. Stamp, Manuel S. Brugger, Achim Wixforth und Christoph Westerhausen
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Sascha Buchegger, Caroline Vogel, Rudolf Herrmann, Bernd Stritzker, Achim Wixforth und Christoph Westerhausen
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Christoph Westerhausen, Lukas G. Schnitzler, Dominik Wendel, Rafał Krzysztoń, Ulrich Lächelt, Ernst Wagner, Joachim O. Rädler und Achim Wixforth
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Melanie E. M. Stamp, Anna Martina Jötten, Patrick W. Kudella, Dominik Breyer, Florian G. Strobl, Thomas M. Geislinger, Achim Wixforth und Christoph Westerhausen
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Melanie E. M. Stamp, Manuel S. Brugger, Achim Wixforth und Christoph Westerhausen
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U. Ratzel, L. Herrmann, Christoph Westerhausen, O. Bayer, K.-G. Krapf, M. Hoffmann, J. Blaul und C. Mehnert
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2015 |
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Melanie E. M. Stamp, Anna Martina Jötten, Dominik Breyer, Mario Djukelic, Florian G. Strobl, Patrick W. Kudella, A. Hartmann, Achim Wixforth und Christoph Westerhausen
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Manuel S. Brugger, M. M. Stamp, Achim Wixforth und Christoph Westerhausen
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2014 |
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Florian G. Strobl, Florian Seitz, Christoph Westerhausen, Armin Reller, Adriano A. Torrano, Christoph Bräuchle, Achim Wixforth und Matthias F. Schneider
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2012 |
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Christoph Westerhausen, Florian G. Strobl, Rudolf Herrmann, A. T. Bauer, S. W. Schneider, Armin Reller, Achim Wixforth und Matthias F. Schneider
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Christoph Westerhausen
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2011 |
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Stephan Niland, Christoph Westerhausen, Stefan W. Schneider, Beate Eckes, Matthias F. Schneider und Johannes A. Eble
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Alexander T. Bauer, Elwira A. Strozyk, Christian Gorzelanny, Christoph Westerhausen, Anna Desch, Matthias F. Schneider und Stefan W. Schneider
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A. Bauer, E. Strozyk, C. Gorzelanny, Christoph Westerhausen, A. Desch, M. F. Schneider und S. W. Schneider
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Lebenslauf
Mehr Publikationen und früherer Blog der Biophysik Gruppe (Stand Februar 2020, Link)
Vita
since 2019: Research group leader "Biophysics Group", Medizinsche Fakultät, Lehrstuhl für Physiologie, University of Augsburg
2018: Research stay at the Karolinska Institute in Stockholm
2014 - 2019: Research group leader, Institute of Physics, Experimentalphysik 1, University of Augsburg
2012 – 2015: Project manager at Wölfel Beratende Ingenieure, Würzburg
2012: Dissertation „On the role of soft interfaces for the understanding of blood clotting”
2011: Research period at Boston University
2009 – 2012: University of Augsburg & University Hospital Mannheim